ABSTRACT
Introduction and Aims: proteinuria means the presence of an excess of serum proteins in the urine that is very important to achieve accurate and reliable results to the physicians. The aim of this study was to evaluate the interference of turbidity in the measurement of 24-hour urine protein before and after cold treatment in turbid centrifuged samples
Materials and Methods: this experimental study was performed in ACECR [Academic Center for Education, Culture and Research], that was conducted on a random sample of 1000 people in 2015 March to September. The measurement of 24-hour urine protein was performed by using three chloral acetic acid method [TCA] and photometer
Results: ten samples [1%] just were turbid after centrifuge. There was a significant difference [P<0.05] between the samples which were turbid after centrifuge and the samples which their turbidity have removed by cold treatment. So that, in some cases the interpretation of the results after cold treatment is quite different with before [the lowest difference was 0.05 and most difference was 0.43]
Conclusion: in order to deposit interfering factors, the urine samples that are taken to measure protein and are turbid after centrifuge should be given cold treatment and centrifuge again